Cell Culture
last authored: March 2010, David LaPierre
last reviewed:
Introduction
Cell culture can be very helpful for in vitro testing.
Primary cell cultures are taken directly from animals. Commonly-used lines include:
- rhesus monkey kidney (influenza virus isolation)
- rabbit kidney (herpes simplex isolation)
Diploid cell culture is derived from fetal tissue fibroblasts. These cell lines have a limited lifespan.
Heteroploid cell lines are derived from neoplastic or transformed cells. These can be grown for many generations.
Commonly used lines include:
- A549 (lung carcinoma)
- HeLa (cervical carcinoma
Maintaining Cell Lines
Temperature
Media
Media contains FBS/FCS.
pH indicators show when media should be changed.
Some labs add antibiotics and antifungals.
Passaging Cells
Resources and References
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