Albert Chezzecook is a 40 year-old man who develops a severe headache, fever, stiff neck, and confusion over 3 days. His family brings him to the emergency department, where he has a seizure.
Suspecting meningitis, the doctor orders a CT scan and performs a lumbar puncture to examine his cerebral spinal fluid (CSF).
Question 1: Which of the following is a common cause of meningitis?
A: Streptococcus pneumoniae
B: Neisseria gonorrhea
C: Staphylococcus aureus
D: Legionella pneumophilia
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Question 2: What do you expect to see in the CSF of an acute bacterial meningitis?
A: high glucose, high protein
B: low glucose, high protein
C: high glucose, low protein
D: low glucose, low protein
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Sir Charles Bell - Opisthotonos 1809 (source)
Question 3: How should samples from sterile sites be processed in the lab for polymerase chain reaction (PCR?)
A: alongside other samples, as usual
B: separate from other samples
C: alongside other samples, though should be rushed (STAT)
D: saved for the end of the day, when the lab is less busy
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Question 4: What pathogen causing meningitis is frequently detected using PCR?
A: Neisseria meningitidis
B: Hemophilus influenza
C: herpes simplex virus
D: rotavirus
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Question 5: How should samples be loaded during PCR?
A: 1- positive control, 2- sample, 3- sample, 4- sample, 5- negative control
B: 1- negative control, 2- sample, 3- sample, 4- sample, 5- positive control
C: 1- negative control, 2- positive control, 3-sample, 4- sample, 5- sample
D: 1- positive control, 2- negative control, 3-sample, 4- sample, 5- sample
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